Figure 5.

Accumulation Levels of Plastid rRNAs in GF-305 Peach Leaves.

(A) to (D) RNA gel blot analyses of RNA preparations hybridized with cDNA probes specific for 23S, 16S, 5S, and 4.5S rRNAs after electrophoresis on denaturing agarose (1.2%) gels for detecting 23S and 16S rRNAs ([A] and [B]) or on denaturing polyacrylamide (5%) gels for detecting 5S and 4.5S rRNAs ([C] and [D]). Only the autoradiogram sections corresponding to each of the four rRNAs, and to a larger precursor observed exclusively in lane 3, are presented.

(E) Ethidium bromide pattern of the upper section of the polyacrylamide gel showing equal intensity of the larger bands generated by the nucleus-encoded 25S and 18S rRNAs. The absence of the smaller bands in the RNA preparation from the albino leaf sector is consistent with the low accumulation of plastid rRNAs detected by RNA gel blot hybridization.

(F) Overexposure of the membrane corresponding to (D) showing the presence of a larger RNA that most likely corresponds to the p23S-4.5S intermediate.