Figure 4.

The eostre Mutant Phenotype Is Caused by a Ds Insertion within the Intergenic Region between the Genes At2g35940 and At2g35950.

(A) Position of the Ds element in eostre-1 and of the T-DNA insertion in eostre-2 and eostre-3. The arrow indicates the GUS gene within the Ds insertion. The Ds insertion is located 11,272 bp upstream of the At2g35940 (BLH1) start codon.

(B) DIC image showing eostre-2 mutant embryo sac phenotype at FG7.

(C) DIC image showing eostre-3 mutant embryo sac phenotype at FG7.

(D) Phenotype of an eostre-2/EOSTRE silique. The insert shows an enlarged section of the silique, and the arrow points to an aborted ovule.

(E) Phenotype of an eostre-3/EOSTRE silique. The insert shows an enlarged section of the silique, and the arrowhead points to an aborted ovule.

Ccn, central cell nucleus; EcL, egg cell–like cell; mi, micropylar pole of the embryo sac; SL, synergid-like cell.