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. 2007 Nov;19(11):3563–3577. doi: 10.1105/tpc.107.053967

Figure 6.

Figure 6.

CCG Interacts with TFIIF through the N Terminal Zinc β-Ribbon Domain in Yeast.

Yeast cells cotransformed with plasmids as indicated at the left side. On top, they were grown on SD/-Leu/-Trp (top panel), SD/-Leu/-Trp/-His (middle panel), or SD/-Leu/-Trp/-His/-Ade (bottom panel). Cell growth on SD/-Leu/-Trp/-His/-Ade indicates the interaction between the two cotransformed gene products. Note CCG N40 interacts strongly with At TFIIF and Sc TFIIF, and there is weaker interaction for CCG and CCGN40-TFIIBN60D chimeric protein with At TFIIF and Sc TFIIF. At TFIIF, Arabidopsis TFIIF; CCGN40, CCG N-terminal 40 amino acids; CCGN40-ScTFIIBN60D, chimeric protein between CCG N-terminal 40 amino acids and S. cerevisiae TFIIB with N-terminal 60–amino acid deletion; Sc TFIIB, S. cerevisiae TFIIB; Sc TFIIBN60D, Sc TFIIB with N-terminal 60–amino acid deletion; Sc TFIIF, S. cerevisiae TFIIF.