Figure 8.

Expression Pattern of the CCG Gene in Wild-Type Plants.

CCG expression pattern revealed by RT-PCR in different organs (A) and floral stages (B) as indicated and ProCCG-GUS-3T transgene ([C] to [G]) and RNA in situ hybridization (H). Bar = 1mm in (C) and (E), 50 μm in (D), and 20 μm in (F) and (H).

(A) RT-PCR results showing CCG expression in seedlings (Sd), inflorescences (In), and siliques (Si) with the ACT2 gene as an internal reference. Rt, root; Le, leaf; St, stem.

(B) RT-PCR results showing CCG expression in flowers at stages FG2-3 (F1), FG4-5 (F2), and FG6-7 (F3) (Christensen et al., 1998). CCG is also expressed in pistils 12 h after emasculation (E12), 48 HAP (H48), and 72 HAP, indicating CCG expression in mature ovules and fertilized ovules.

(C) A micrograph of the transgenic root showing CCG expression in root tip.

(D) A micrograph showing CCG expression in the shoot apex (arrow).

(E) A micrograph showing CCG expression in anthers.

(F) A Nomarski micrograph of an ovule at FG6 showing CCG expression in the central cell (arrow) of the ovule.

(G) A Nomarski micrograph of an ovule at FG7 showing CCG expression in the central cell (arrow; Cc). Note no expression in egg cell (Eg) and synergids (Sy).

(H) An oblique section of an ovule at FG7 hybridized with CCG antisense RNA probe showing CCG expression in the central cell.