Table 1.
No. of Female Gametophytes at Developmental Stages
|
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Pistil No. | FG0 | FG1 | FG2 | FG3 | FG4 | FG5 | FG6 | FG7 |
1 | 33 | |||||||
2 | 30 | 2 | ||||||
3 | 5 | 17 | ||||||
4 | 10 | 16 | 4 | |||||
5 | 4 | 15 | 1 | |||||
6 | 3 | 19 | 2 | |||||
7 | 5 | 28 | 8 | |||||
8 | 9 | 11 | ||||||
9 | 2 | 6 | 11 | |||||
10 | 12 | 18 | ||||||
11 | 6 | 17 | ||||||
12 (Em7 h) | 3 | 29 |
Ovules from 12 pistils of the same inflorescence were analyzed by confocal microscopy, and their developmental stages (FG0 to FG7) were determined according to Christensen et al. (1998). Ovules within each pistil are mostly at two adjacent developmental stages, the same as that in wild-type plants described by Christensen et al. (1998) and Shi et al. (2005), indicating that the mutation does not affect developmental synchrony. Em7 h, 7 h after emasculation.