Figure 5.

Domain Analysis of the MS1 Protein.

Lane 1 shows a construct in which MS1 cDNA was driven by the CaMV 35S promoter. Instead of CaMV 35S, MS1 promoter was used in the constructs in lanes 2 to 6. MS1(WT) is a construct expressing wild-type MS1 cDNA. MS1(Δ212-304) is an internal deletion mutant. The deleted region (amino acids 212 to 304) contains the Leu zipper–like region. MS1(E627K), MS1(C635A), and MS1(H640N, C643G) are amino acid residue–substituted mutants in the PHD region. NLS, LZ, and PHD shown by dark gray indicate nuclear localization signal, Leu zipper–like region, and PHD region, respectively. Fertile: fertilities of this column were equivalent to those of wild-type Ler plants. Semi-sterile: plants of this column were less fertile than wild-type Ler plants. Completely sterile: plants of this column produced no seeds.