Table 1.
Selected Genes Whose Expression Was Affected by the ms1 Mutation, from the Microarray Data of Yang et al. (2007)
| Transcript IDa | Probe Set IDa | DEX Inductionb | TargetP Predictionc | Gene Titlea | Possible Functiond | ms1 Younge | ms1 Olde | Ler Youngf | Ler Oldf |
|---|---|---|---|---|---|---|---|---|---|
| At1g06260 | 260788_at | Examined | S | Cys proteinase, putative | Protease | 28.1 ± 6.7 | 44.6 ± 13.8 | 1418.5 ± 349.8 | 222.8 ± 134.1 |
| At1g06990 | 256059_at | Examined | S | GDSL-motif lipase/hydrolase family protein | 4.6 ± 5.3 | 9.1 ± 9.0 | 152.3 ± 54.4 | 39.6 ± 23.5 | |
| At1g13140 | 262764_at | Examined | Cytochrome P450 family protein | Lipid, CYP86C3 | 24.2 ± 10.1 | 20.1 ± 8.4 | 647.0 ± 240.2 | 55.6 ± 42.9 | |
| At1g13150 | 262765_at | ND | S | Cytochrome P450, putative | Lipid, CYP86C4 | 13.0 ± 9.5 | 14.4 ± 10.6 | 301.4 ± 132.4 | 37.2 ± 19.1 |
| At1g23240 | 262987_at | ND | Caleosin-related family protein | PC | 9.5 ± 4.6 | 34.7 ± 38.0 | 404.4 ± 139.3 | 306.1 ± 205.4 | |
| At1g23250 | 263011_at | Examined | Caleosin-related | PC | 3.1 ± 3.2 | 4.5 ± 4.3 | 71.0 ± 49.1 | 34.3 ± 22.2 | |
| At1g28430 | 261499_at | ND | S | Cytochrome P450, putative | CYP705A24 | 7.9 ± 4.6 | 21.8 ± 11.1 | 300.3 ± 118.4 | 216.5 ± 184.2 |
| At1g61110 | 264882_at | Examined | No apical meristem (NAM) family protein | TF | 6.2 ± 2.0 | 10.5 ± 5.5 | 713.6 ± 262.8 | 69.4 ± 49.4 | |
| At1g67990 | 260001_at | ND | Caffeoyl-CoA 3-O-methyltransferase, putative | PP | 7.1 ± 0.4 | 12.8 ± 9.6 | 1501.5 ± 300.6 | 167.0 ± 195.1 | |
| At1g71160 | 259744_at | ND | S | β-Ketoacyl-CoA synthase family protein | Lipid | 25.9 ± 6.0 | 9.5 ± 2.7 | 225.2 ± 81.0 | 21.5 ± 21.2 |
| At1g74540 | 260228_at | Examined | Cytochrome P450, putative | PP, CYP98A8 | 5.5 ± 2.8 | 2.4 ± 0.1 | 498.8 ± 225.3 | 26.2 ± 31.8 | |
| At1g74550 | 260233_at | ND | Cytochrome P450, putative | PP, CYP98A9 | 40.7 ± 16.7 | 38.1 ± 5.2 | 353.3 ± 144.6 | 54.6 ± 15.6 | |
| At1g75910 | 262674_at | ND | S | Family II extracellular lipase 4 (EXL4) | PC | 20.2 ± 12.0 | 28.3 ± 19.1 | 1893.7 ± 591.5 | 275.1 ± 181.5 |
| At1g75920 | 262683_at | Examined | S | Family II extracellular lipase 5 (EXL5) | PC | 11.6 ± 6.1 | 17.9 ± 11.3 | 540.7 ± 215.7 | 89.1 ± 55.4 |
| At1g75930 | 262675_at | ND | S | Family II extracellular lipase 6 (EXL6) | PC | 3.3 ± 2.0 | 61.4 ± 56.9 | 1226.9 ± 351.7 | 564.7 ± 350.4 |
| At1g75940 | 262697_at | Examined | S | Glycosyl hydrolase family 1 protein/anther-specific protein ATA27 | 61.5 ± 11.7 | 56.7 ± 9.0 | 1830.9 ± 413.0 | 308.0 ± 243.5 | |
| At1g76470 | 259975_at | ND | Cinnamoyl-CoA reductase family | PP | 22.4 ± 6.9 | 19.6 ± 6.3 | 282.2 ± 129.8 | 32.5 ± 22.6 | |
| At2g03200 | 266708_at | Examined | Aspartyl protease family protein | Protease | 4.5 ± 4.3 | 24.8 ± 11.9 | 266.1 ± 76.9 | 38.4 ± 18.1 | |
| At2g19070 | 267440_at | Examined | Transferase family protein | PP | 8.3 ± 2.4 | 7.3 ± 2.1 | 382.0 ± 194.1 | 26.3 ± 18.3 | |
| At2g23800 | 267295_at | Examined | S | Geranylgeranyl pyrophosphate synthase (GGPS2) (GGPS5)/GGPP synthetase/farnesyltranstransferase | PT | 11.0 ± 14.9 | 36.4 ± 26.5 | 389.1 ± 193.9 | 246.6 ± 178.9 |
| At3g26125 | 257633_at | ND | Cytochrome P450, putative | Lipid, CYP86C2 | 64.6 ± 18.7 | 138.4 ± 45.1 | 849.0 ± 290.1 | 254.3 ± 166.7 | |
| At3g51590 | 252063_at | Examined | S | Lipid transfer protein, putative | Lipid | 22.1 ± 21.7 | 12.5 ± 8.8 | 1827.2 ± 484.9 | 225.4 ± 211.5 |
| At3g52160 | 252035_at | Examined | β-Ketoacyl-CoA synthase family protein | Lipid | 21.0 ± 7.4 | 13.5 ± 0.9 | 496.2 ± 223.9 | 45.3 ± 31.8 | |
| At3g57810 | 251558_at | ND | OTU-like Cys protease family protein | Protease | 108.8 ± 17.3 | 98.1 ± 11.4 | 487.3 ± 213.6 | 224.9 ± 92.1 | |
| At4g22080 | 254338_s_at | Examined | S | Pectate lyase family protein | 0.4 ± 0.4 | 0.1 ± 0.1 | 87.2 ± 36.5 | 7.0 ± 11.8 | |
| At4g22090 | |||||||||
| At4g23660 | 254230_at | ND | M | UbiA prenyltransferase family protein | PT | 49.2 ± 9.5 | 54.9 ± 3.5 | 297.8 ± 89.9 | 83.2 ± 23.0 |
| At4g28395 | 253772_at | Examined | Lipid transfer protein, putative | Lipid | 30.4 ± 6.4 | 11.6 ± 4..8 | 986.3 ± 249.3 | 69.8 ± 71.0 | |
| At5g07510 | 250635_at | ND | S | Gly-rich protein (GRP14) | PC | 7.4 ± 5.2 | 35.0 ± 7.5 | 422.6 ± 167.2 | 151.3 ± 81.5 |
| At5g07520 | 250636_at | ND | S | Gly-rich protein (GRP18) | PC | 9.9 ± 2.7 | 33.3 ± 8.3 | 491.3 ± 317.7 | 166.3 ± 105.5 |
| At5g07530 | 250637_at | Examined | Gly-rich protein (GRP17) | PC | 10.3 ± 0.4 | 119.5 ± 122.9 | 1344.0 ± 603.8 | 706.2 ± 455.9 | |
| At5g07540 | 250638_at | ND | S | Gly-rich protein (GRP16) | PC | 26.4 ± 12.1 | 41.6 ± 9.1 | 636.4 ± 322.5 | 232.2 ± 143.7 |
| At5g07550 | 250610_at | ND | S | Gly-rich protein (GRP19) | PC | 25.7 ± 20.3 | 337.8 ± 422.8 | 2450.7 ± 447.5 | 1502.9 ± 889.6 |
| At5g07560 | 250639_at | ND | Gly-rich protein (GRP20) | PC | 8.1 ± 6.6 | 85.3 ± 62.6 | 1856.4 ± 411.9 | 786.0 ± 519.3 | |
| At5g13380 | 250294_at | Examined | Auxin-responsive GH3 family protein | 9.0 ± 3.2 | 10.7 ± 7.6 | 403.1 ± 146.1 | 34.9 ± 34.5 | ||
| At5g49070 | 248638_at | ND | β-Ketoacyl-CoA synthase family protein | Lipid | 14.2 ± 9.8 | 9.3 ± 2.0 | 112.8 ± 49.0 | 15.1 ± 5.6 | |
| At5g60500 | 247639_s_at | Examined | Undecaprenyl pyrophosphate synthetase family protein/UPP synthetase family protein | PT | 1.3 ± 1.5 | 1.4 ± 0.1 | 154.8 ± 111.5 | 12.0 ± 12.9 | |
| At5g60510 | |||||||||
| At5g62320 | 247451_at | Examined | Myb family transcription factor (MYB99) | TF | 9.2 ± 2.1 | 9.6 ± 5.1 | 119.6 ± 41.2 | 16.4 ± 7.9 |
We used publicly available NASCArray data (experiment ID: NASCARRAYS-23) at http://affymetrix.Arabidopsis.info/. After eliminating genes with signal values <15.0 in Ler young, we selected genes with more than fivefold higher expression in Ler young than in ms1 old. Among them, we further selected genes with more than threefold higher expression in Ler young than in ms1 young. In total, we isolated 95 genes. Here, we show selected 39 genes among them. The complete list is shown in Supplemental Table 1 online.
These columns are the same as the original TIGR ATH1-121501 database.
Thirty-two genes out of 95 were examined for DEX inducibility using the DEX induction system as shown in Figure 6C and Supplemental Figure 1 online. See Supplemental Table 1 online for the complete list. ND, not determined.
Potential subcellular localization signal was predicted using the TargetP program (Emanuelsson et al., 2000; http://www.cbs.dtu.dk/services/TargetP/). Localization to the secretory pathway (S) and mitochondrion (M) with prediction reliability class 1 to 3 is indicated.
Possible function of a gene product involved in lipid biosynthesis and transfer (lipid), phenylpropanoid biosynthesis (PP), pollen coat protein (PC), cytochrome P450 family (CYP), protease, transcription factor (TF), or prenyltransferase (PT).
These columns show average of signal values (± sd) from three independent hybridization slides done by NASCArray.
These columns show average of signal values (± sd) from five independent hybridization slides done by NASCArray.