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Detection by DNA Gel Blot of the Bleomycin Resistance Marker and the NIT2 Gene in the Parental Strain 704 and AE Mutants.
Genomic DNA (5 μg) of the indicated strains was digested with the indicated restriction enzymes and hybridized with a 1.2-kb XhoI fragment from plasmid pSP124S (BLE) (A) or with a 1.5-kb HindIII-SalI fragment of plasmid pMN68 of the NIT2 gene (B).
