Figure 2.

Developmental Abnormalities of ago1, fry1, and xrn Mutants.

(A) and (B) ago1-27 and ago1-33 mutant lines used for mutagenesis. Plants are dwarf and have serrated leaves.

(C) to (F) fry1-4 and fry1-5 mutants recovered from the mutagenesis screen are shown in the ago1 mutant backgrounds ([C] and [D]) or alone ([E] and [F]). Plants are dwarf and have crinkly leaves, rounded leaf margins, and delayed flowering compared with wild-type plants.

(G) A Columbia (Col) accession wild-type plant.

(H) fry1-6 T-DNA insertion mutants display developmental anomalies identical to fry1-4 and fry1-5 mutants.

(I) xrn2-1 mutants do not display obvious developmental defects.

(K) Hypomorphic xrn3-3 mutants display crinkly leaves and delayed flowering.

(M) xrn4-6 mutants display slightly serrated leaves.

(J), (L), and (N) xrn2-1 xrn3-3 (J), xrn2-1 xrn4-6 (L), and xrn3-3 xrn4-6 (N) double mutants show additive developmental defects, and xrn2-1 xrn3-3 mutants (J) resemble fry1-4, fry1-5, and fry1-6 single mutants ([E], [F], and [H], respectively).