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. 2007 Nov;19(11):3451–3461. doi: 10.1105/tpc.107.055319

Figure 3.

Figure 3.

ago1 fry1 Double Mutants Posttranscriptionally Silence L1.

RNA gel blot analysis of GUS mRNA (A) and GUS siRNA (B) accumulation in L1 control and ago1, fry1, and ago1 fry1 mutant 18-d-old seedlings.

(A) L1 control and L1/fry1-4 and L1/fry1-5 mutant plants are silenced and accumulate low GUS mRNA levels. ago1-27 and ago1-33 mutants release L1 silencing and accumulate high levels of GUS mRNA. Both fry1-4 and fry1-5 restore L1 silencing in ago1 mutant backgrounds and accumulate low levels of GUS mRNA, although L1 silencing is restored to a lesser extent in L1/ago1-27/fry1-4 mutants (4.2 versus 1.3). Normalized values of GUS mRNA to ethidium bromide–stained 25S RNA (bottom panel), with L1 levels set at 1.0, are indicated.

(B) RNA gel blot analysis of GUS siRNA accumulation. L1 control, L1/fry1-4, L1/fry1-5, L1/ago1-27/fry1-4, and L1/ago1-33/fry1-5 mutant plants are silenced and accumulate GUS siRNAs. ago1-27 and ago1-33 mutants release L1 silencing and do not accumulate detectable GUS siRNAs. Blots were stripped and rehybridized with a probe complementary to U6 snRNA as a loading control. Normalized values of GUS siRNAs to U6 small nuclear RNA (snRNA), with L1 levels set at 1.0, are indicated. ND, not detected.