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. 2007 Nov;19(11):3451–3461. doi: 10.1105/tpc.107.055319

Figure 4.

Figure 4.

fry1 Mutants Overaccumulate miRNA Target 3′ Cleavage Products.

RNA gel blot analysis of AGO1 full-length mRNA and miR168 generated 3′ AGO1 cleavage products, and ARF10, ARF16, and ARF17 full-length RNA and miR160 generated 3′ ARF cleavage products in L1 control and ago1, fry1, ago1 fry1, xrn2, xrn3, and xrn4 mutant 18-d-old seedlings.

(A) AGO1 full-length mRNA levels are increased in ago1 and ago1/fry1 mutants, whereas AGO1 3′ cleavage products, but not AGO1 full-length mRNA, overaccumulate in fry1 single mutants.

(B) and (C) fry1 mutants and ago1 fry1 double mutants overaccumulate ARF10, ARF16, and ARF17 3′ cleavage products (B), similar to xrn4 mutants (C). Ethidium bromide–stained 25S RNA is shown as a loading control.