Figure 1.

Transcriptional Inhibition after Treatment with ActD in Arabidopsis Cell Culture.

(A) Transcript abundance of some genes analyzed by both QRT-PCR and microarray analysis with the calculated correlation coefficient given for each transcript. A value of 1.0 was assigned to the level of transcript prior to treatment, and the transcript abundance at all other time points was expressed relative to this value. Each data point represents the mean ± se. The right panel displays transcript abundance of the same genes analyzed by QRT-PCR following mock treatment with ethanol.

(B) Examples of mRNA decay profiles (using the microarray data) and the nonlinear least-squares regression curve produced for each gene. Data were regressed using the relative values from all three biological replicates over all the time points using SAS, which allowed the rate of decay for each gene to be determined. The blue line represents the actual data points, and the red line represents the fitted exponential model.

WRKY6, WRKY6 transcription factor (At1g62300); OM64, outer mitochondrial membrane protein of 64 kD (At5g09420); MDHAR, monodehydroascorbate reductase (At1g63940); UCP, uncoupling protein (At3g54110); HPR, hydroxypyruvate reductase (At1g68010); TIM14, translocase of the inner membrane protein 14 (At2g35795).