Figure 6.

Comparison of the Patterns of Transcript Changes Observed upon Treatment with ActD and Several Stresses.

(A) SOM analyses of transcript abundance data from microarray analysis following ActD treatment. The transcripts were grouped by a 1 × 5 SOM (labeled C1-C5) using GeneCluster 2.0 software. In each of the graphs, the solid blue line represents the data mean, and the red lines indicate the range of the data fitted to form each cluster. For each of the clusters, n = the number of genes in that cluster, A = the number of decay profiles (following ActD treatment), and SCA = the number of genes whose expression profiles under one or more stress(es) clustered with their respective ActD decay profile. This produced a list of 711 transcripts (total number of SCA) whose expression profile, under at least one stress, clustered with the respective ActD decay profile of that transcript.

(B) Heat map of the mRNA decay profiles and stress transcript expression profiles that clustered together. Rapidly decaying transcripts can be seen by a change from red to green at the early time points (indicated in hours at the top of the heat map). An increase in the brightness of green indicates a decrease in transcript abundance. By contrast, slow-decaying transcripts only changed from red to green at later times. For each gene, the transcript abundance at 0 h was set to 1, and the transcript abundance at all other times were expressed in a relative manner. Next to the heat map is a colored distribution representing the six most abundant functional categories in decreasing order from left to right.

(C) The 12 transcripts that were found to cluster with three or more stresses. The transcripts that encode transcription factors are indicated in red.