Figure 1.

Analysis of the ppcA GUS Reporter Gene Constructs FtPR, FtM-FtPR, and FbM-FtPR in Transgenic F. bidentis.

(A) Schematic presentation of the ppcA GUS chimerical genes. Nucleotide numbers refer to the translation initiation codon. The Ft MEM1 region is indicated by light green boxes, and the proximal region (PR) is indicated by dark green boxes. The state of the C₃/C₄-associated polymorphisms in the A submodule (G or A) and the B submodule (presence or absence of CACT) of MEM1 are indicated.

(B) GUS activities in leaves of transgenic F. bidentis plants. The median values (black bars) of the GUS activities are expressed in nanomoles of the reaction product 4-methylumbelliferone (MU) generated per milligram of protein per minute. The numbers of independent transgenic plants investigated (N) are indicated at the top of each column.

(C) to (E) Histochemical localization of GUS activity in leaf sections of transgenic F. bidentis plants transformed with the FtPR (C), FtM-FtPR (D), or FbM-FtPR (E) construct. Incubation times were 25 h (C), 12 h (D), and 24 h (E).