Figure 2.

Analysis of the ppcA GUS Reporter Gene Constructs ppcAFp, FpM-FpPR, FpM-FtPR, FtM/A_ΔCACT-FtPR, and FpM/G_+CACT-FtPR in Transgenic F. bidentis.

(A) Schematic presentation of the ppcA GUS chimerical genes. Nucleotide numbers refer to the translation initiation codon. The Ft MEM1 region is indicated by light green boxes, and the Fp MEM1 region is indicated by light yellow boxes. The Ft proximal region (PR) is indicated by dark green boxes, and the Fp PR is indicated by dark yellow boxes. The state of the C₃/C₄-associated polymorphisms in the A submodule (G or A) and the B submodule (presence or absence of CACT) of MEM1 are indicated.

(B) GUS activities in leaves of transgenic F. bidentis plants. The numbers of independent transgenic plants investigated (N) are indicated at the top of each column. Median values (black bars) are shown. MU, 4-methylumbelliferone.

(C) to (G) Histochemical localization of GUS activity in leaf sections of transgenic F. bidentis plants transformed with ppcAFp (C), FpM-FpPR (D), FpM-FtPR (E), FtM/A_ΔCACT-FtPR (F), and FpM/G_+CACT-FtPR (G). Incubation times were 2 h (C), 3.5 h (D), 18 h (E), 17 h (F), and 3 h (G).