Table 1.
Oligonucleotides Used for the Generation of MEM1 Reporter Gene Vectors
| Promoter–GUS-Reporter Gene Vectors | Oligonucleotides/Primers | Sequences (5′ to 3′) |
|---|---|---|
| FtM/Δnt11-FtPR | FtDEΔAB5′HindIII | AGCTTAGAGCTGTACTTACTCACTAAAACAAACAAT |
| FtDEΔAB3′XbaI | CTAGATTGTTTGTTTTAGTGAGTAAGTACAGCTCTA | |
| FtM/Δnt12-41-FtPR | FtDEAΔB5′HindIII | AGCTTGTGAATTTATGT |
| FtDEAΔB3′XbaI | CTAGACATAAATTCACA | |
| FtM/A-FtPR | FtDEaB5′HindIII | AGCTTATGAATTTATGAGAGCTGTACTTACTCACTAAAACAAACAAT |
| FtDEaB3′XbaI | CTAGATTGTTTGTTTTAGTGAGTAAGTACAGCTCTCATAAATTCATA | |
| FtM/ΔCACT-FtPR | FtDEAb5′HindIII | AGCTTGTGAATTTATGAGAGCTGTACTTACTAAAACAAACAAT |
| FtDEAb3′XbaI | CTAGATTGTTTGTTTTAGTAAGTACAGCTCTCATAAATTCACA | |
| FtM/A_ΔCACT-FtPR | FtDEab5′HindIII | AGCTTATGAATTTATGAGAGCTGTACTTACTAAAACAAACAAT |
| FtDEab3′XbaI | CTAGATTGTTTGTTTTAGTAAGTACAGCTCTCATAAATTCATA | |
| FbM-FtPR | FbDEAB5′HindIII | GGGAAGCTTGTGAATTTATGAAAAAATTAAATTGGAAAGAGG |
| FbDEAB3′XbaI | GGGTCTAGATTGTTTGTTTTAGTGAGTAAGTACGGCTCTACGAACAC | |
| ppcAFp | Fp5′HindIII | GGGAAGCTTTTTCTTTTGTATTTGTTATTGTTTACG |
| Fp3′HpaI | GGGGTTAACGCCTCTATGTACAGAGAATACC | |
| FpM-FpPR FpM-FtPR | FpDEab5′HindIII | GGGAAGCTTATGAATTTATGAAAAACTCGTG |
| FpDEab3′XbaI | GGGTCTAGATTGTTTGTTTTAGTAAGTACG | |
| FpM/G_+CACT-FtPR | FpDEAB5′HindIII | GGGAAGCTTGTGAATTTATGAAAAACTCGTGAAGAG |
| FbDEAB3′XbaI | GGGTCTAGATTGTTTGTTTTAGTGAGTAAGTACGGCTCTACGAACAC |
Restriction sites of HindIII, XbaI, and HpaI are given in boldface letters.