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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Microbiol Methods. 2007 Sep 21;71(3):332–335. doi: 10.1016/j.mimet.2007.09.006

Figure 1.

Figure 1

Transposon delivery plasmids. A. pAW016, the mini-Tn10 delivery plasmid. CmR, chloramphenicol-resistance cassette; SpcR, spectinomycin-resistance cassette; IR, invert repeat transposase recognition sites. Vector constructed from source plasmids as indicated in text. B. pAW068, the Mariner transposon delivery plasmid. C. Features of the Mariner transposon sequence in greater detail showing the relative location of the pUC origin of replication, spectinomycin resistance cassette, flanking FLP recombinase target (FRT) sites, and the 3xFLAG epitope tag.