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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Virus Res. 2007 Jun 20;130(1-2):269–274. doi: 10.1016/j.virusres.2007.05.012

Figure 2.

Figure 2

Immunofluorescence (IF) showing that MAb 3015B2 is reacting specifically to VACV E3 protein. RK-13 cells seeded on an 8-chamber glass microscope slide (Nunc) were either untransfected or transfected for 24 hours with 2 μg of pMT-E3L using FuGene 6 reagent (Roche). Cells were then either left uninfected or infected with WR or WRΔE3L viruses for 24 hours. Cells were then fixed with 4% formalin, permeabilized with 0.1% Triton X-100 in PBS, and then probed with hybridoma generated mouse ascites at a 1:200 dilution. Cells were then washed with 3% bovine serum albumin (3% BSA-PBS), probed with goat anti-mouse immunoglobulin G conjugated to fluorescein isothiocyanate (FITC) (Zymed) at a 1:200 dilution, and washed with PBS. The nuclei of the cells were stained with DAPI (Vector Labs). Cells were visualized using a Nikon Eclipse E1000 fluorescence microscope with the appropriate filters and analyzed using Phase 3 Imaging software.