Figure 2.

Effect of 50ng of D-Phe-CRH in mPFC on ACTH and corticosterone responses to acute or repeated stress. Plasma ACTH and plasma corticosterone responses to the first restraint (acute restraint) or to the eighth restraint (repeated restraint) are shown in rats that received intra-mPFC injections of 50ng of D-Phe-CRH or VEH 30min prior to the 1st or 8th restraint. (The following are sample sizes at each time point of the ACTH response. For acute vehicle, n=8 at 0 min, n=9 at 15 min, n=11 at 30 min, and n=10 at 60 min. For acute drug, n=7 at 0 min, n=8 at 15 min, n=10 at 30 min, n=9 at 60 min. For repeated vehicle, n=7 at 0 min and 15 min, n=12 at 30 min, and n=10 at 60 min. Lastly, for repeated drug, n=8 at 0 min, n=7 at 15 min, n=12 at 30 min, and n=10 at 60 min. The following are sample sizes at each time point of the corticosterone response. For acute vehicle, n=11 at 0 min, n=14 at 15 min, n=13 at 30 min, and n=7 at 60 min. For acute drug, n=11 at 0 min, n=14 at 15 min and 30 min, and n=10 at 60 min. For repeated vehicle, n=12 at 0 min, n=14 at 15 min, n=13 at 30 min, and n=14 at 60 min. Lastly, for repeated drug, n=8 at 0 min, n=12 at 15 min, n=9 at 30 min, and n=15 at 60 min.)

*D-Phe-CRH-injected groups are significantly lower than vehicle-injected groups regardless of whether animals were acutely or repeatedly stressed, reflecting a significant Main effect of Drug Treatment. p≤ 0.05.

** Repeatedly-stressed groups are significantly lower than acutely-stressed groups regardless of drug treatment, reflecting a significant Main effect of Stress Group, p≤ 0.05.