Figure 1. Mutation of the Rit effector loop alters neurite outgrowth ability.
(A) Sequence alignment of the effector domains from several Ras family GTPases. (B) PC6 cells were transiently transfected, subjected to G418 (400 μg/ml) selection for 3 days, fixed and stained with FITC-phalloidin to label actin filaments. Representative fluorescent micrographs from random fields are presented. (C) The percentage of neurite-bearing cells was calculated at day 3 and is presented relative to RitL79 (100%) as means ± standard error from three independent experiments.