Figure 2. Mutation of the Rit effector loop alters activation of downstream MAPK pathways.

PC6 cells were transiently transfected with expression vectors for the indicated Rit mutant, subjected to serum starvation, and harvested as described under “Materials and Methods”. 50 μg of pre-cleared lysate was resolved on SDS 10%-polyacrylamide gels and the levels of activated ERK1/2 and p38 MAPK were determined by immunoblotting with the appropriate phospho-specific antibodies. Blots are representative of three independent trials.