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. 2000 Apr 3;149(1):209–222. doi: 10.1083/jcb.149.1.209

Figure 1.

Figure 1

(a) Schematic representation of plakophilin 1 (PKP-1) and its deletion mutant constructs used in this study. The PKP-1 head comprises amino acids 1–286; PKP-1 ΔC1, amino acids 1–213; PKP-1 ΔC2, amino acids 1–168; PKP-1 ΔN1, amino acids 70–286; and PKP-1 ΔN2, amino acids 147–286. The PKP-1 arm repeat domain contains amino acids 287–726, and the PKP-1 headless fragment contains amino acids 224–726. The ΔENCMC fragments carry an additional internal deletion comprising amino acids 422–426. (b) Specificity of plakophilin head and arm repeat domain antibodies. Total protein extracts (lanes 1 and 5) as well as Triton-soluble (lanes 2 and 6), high salt soluble (lanes 3 and 7), and insoluble fractions (lanes 4 and 8) of HaCaT keratinocytes were separated on 8% SDS gels, transferred to nitrocellulose, and probed with the plakophilin head (lanes 1′–4′) and repeat domain antibodies (lanes 5′–8′). Both antibodies reacted with a single band of 80 kD in total cell extracts (lanes 1′ and 5′). The majority of the protein was in the insoluble fraction (lanes 4′ and 8′).