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. 2000 Apr 3;149(1):223–236. doi: 10.1083/jcb.149.1.223

Figure 7.

Figure 7

Embryonic fibroblast cells deficient in CAS show reduced migration and survival in 3-D collagen. (A) Migration of embryonic fibroblast cells isolated from wild-type (CAS +/+) or CAS-deficient mice (CAS −/−) for 6 h in buffer (NT) or buffer with FBS (10%). The number of migrating cells invading into the lower chamber was counted as described in Materials and Methods. Results are the mean ± SEM of three independent experiments. (B) Cells treated as for the migration experiment above and examined for apoptosis after 24 h of culture in 3-D collagen in the presence of various concentrations of FBS. The percentage of apoptotic cells was determined by morphological criteria as described in Materials and Methods. Results are the mean ± SEM of three independent experiments. (C) CAS −/− cells transiently transfected with the empty vector (Mock) or the vector containing wild-type CAS (CAS −/− plus CAS), along with a β-gal reporter construct. The number of migratory cells invading into the lower chamber was detected with X-gal and counted as described in Materials and Methods. Results are the mean ± SEM of three independent experiments. CAS immunoblotted from an aliquot of mock (lane 1) or CAS −/− plus CAS transfected cells (lane 2) is also shown. (D) Cells from the migration experiment in C are examined for apoptosis after 24 h of culture in 3-D collagen in the presence or absence of FBS (10%). The percentage of apoptotic cells was determined by morphological criteria as described in Materials and Methods. Results are the mean ± SEM of three independent experiments.