Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Apr 3;149(1):181–194. doi: 10.1083/jcb.149.1.181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

(a) Fyb/SLAP coimmunoprecipitates with WASP. Lysates of activated Jurkat T cells were incubated with the WASP-specific antiserum Fus3. Immune complexes were collected using protein A–agarose. Bound proteins were resolved by SDS-PAGE, blotted, and probed with polyclonal antibodies #51 to Fyb/SLAP. (lane 1) Cell extract of activated Jurkat T cells; (lane 2) immunoprecipitation with a nonimmune serum; and (lane 3) immunoprecipitation using a WASP-specific antiserum. (b) Fyb/SLAP, SLP-76, Nck, and WASP form a multiprotein complex in Jurkat T cells. Lysates from nonstimulated (NST) or stimulated (ST) Jurkat T cells were immunoprecipitated using the WASP mAb #67B4, resolved by SDS-PAGE, blotted, and probed with antibodies to Fyb/SLAP, SLP-76, and Nck. As negative control, lysates were immunoprecipitated using an antibody to a bacterial protein. To exclude nonspecific trapping, WASP immunoprecipitated from lysates of activated T cells were probed using a zyxin mAb. (c) The stimulation of Jurkat T cells results in the association of additional tyrosine-phosphorylated proteins with the complex. WASP immunoprecipitates from lysates of nonstimulated (NST) or stimulated (ST) Jurkat T cells were resolved by SDS-PAGE and probed with an antibody to phosphotyrosine. Note the appearance of three additional bands in stimulated T cells. hc, heavy chain; lc, light chain. Molecular weight markers are indicated on the left in (a and b) and on the right in (c).