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. 2000 Apr 3;149(1):41–54. doi: 10.1083/jcb.149.1.41

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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A pool of Sec13p-GFP localizes to the nuclear pores. (a) The sec13 ⁻ /nup133 ⁻ double mutant complemented by either SEC13-GFP and NUP133 (sec13::HIS3) or by SEC13-GFP alone (nup133::HIS3) was analyzed by fluorescence microscopy (upper panel). In addition, Spo7p-GFP (Siniossoglou et al. 1998) and GFP-Nup49p were tested in the nup133 ⁻ cells (upper panel). For comparison, the seh1::HIS3 disruption mutant complemented by plasmid-borne SEH1-GFP was analyzed in the fluorescence microscope (lower panel). (b) The sec13 ⁻ /nup133 ⁻ double mutant expressing Sec13p-GFP was analyzed by indirect immunofluorescence microscopy using a monoclonal anti-Nsp1p antibody. It was also stained for DNA.