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. 2000 Apr 3;149(1):41–54. doi: 10.1083/jcb.149.1.41

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© 2000 The Rockefeller University Press

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graphic file with name JCB9910076.f3a.jpg — Genetic interaction between the sec13-34 and nup85Δ mutant alleles. (a) Growth curves of the sec13-34/nup85Δ double mutant and of the corresponding sec13-34 and nup85Δ single mutants at 23 and 32°C. The nup85Δ allele is derived from a partial gene disruption and expresses a Nup85p protein which is NH₂-terminally truncated (Siniossoglou et al. 1996). (b) Affinity-purification of Sec13p-ProtA from NUP85/SEC13, nup85Δ/SEC13, and nup85Δ/sec13-14 strains grown at 23°C or shifted for 12 h to 32°C. The purified wild-type or mutant Sec13p-ProtA fusion proteins were analyzed by Western blotting using anti-Nup84p antibodies. Indicated are the positions of Sec13p-ProtA and Nup84p.

graphic file with name JCB9910076.f3b.jpg — Genetic interaction between the sec13-34 and nup85Δ mutant alleles. (a) Growth curves of the sec13-34/nup85Δ double mutant and of the corresponding sec13-34 and nup85Δ single mutants at 23 and 32°C. The nup85Δ allele is derived from a partial gene disruption and expresses a Nup85p protein which is NH₂-terminally truncated (Siniossoglou et al. 1996). (b) Affinity-purification of Sec13p-ProtA from NUP85/SEC13, nup85Δ/SEC13, and nup85Δ/sec13-14 strains grown at 23°C or shifted for 12 h to 32°C. The purified wild-type or mutant Sec13p-ProtA fusion proteins were analyzed by Western blotting using anti-Nup84p antibodies. Indicated are the positions of Sec13p-ProtA and Nup84p.