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The growth of R-41 mutant cells is dependent on PE or its precursor. a, CHO-K1 and R-41 mutant cells were cultured in normal medium containing 10% newborn calf serum at 39.5°C. b, CHO-K1 and R-41 mutant cells were cultured in ethanolamine-deficient medium at 39.5°C (CHO-K1, R-41), and R-41 mutant cells were cultured in either 30 μM PE (R-41+PE) or 20 μM ethanolamine-supplemented medium (R-41+Etn) at 39.5°C.
