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. 2000 Jun 12;149(6):1215–1224. doi: 10.1083/jcb.149.6.1215

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© 2000 The Rockefeller University Press

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graphic file with name JCB0002104.f4a.jpg — R-41 mutant cells have a defect in cytokinesis. A, Morphology of CHO-K1 (a) and R-41 mutant cells (b–d) cultured in normal medium. CHO-K1 and R-41 mutant cells were cultured in normal medium containing 10% newborn calf serum supplemented with (d) or without (a–c) 20 μM ethanolamine at 39.5°C for 3 d. B, Flow cytometric analyses of the DNA contents. CHO-K1 and mutant cells were cultured in ethanolamine-deficient medium supplemented with (R-41+Etn) or without (CHO-K1, R-41) 20 μM ethanolamine at 39.5°C for 4 d. CHO-K1: G₁, 41.6%; S, 49.1%; G₂/M, 9.3%. R-41: G₁, 38.9%; S, 16.3%; G₂/M, 44.8%. R-41+Etn: G₁, 38.2%; S, 50.5%; G₂/M, 11.3%. Bars, 30 μm.

graphic file with name JCB0002104.f4b.jpg — R-41 mutant cells have a defect in cytokinesis. A, Morphology of CHO-K1 (a) and R-41 mutant cells (b–d) cultured in normal medium. CHO-K1 and R-41 mutant cells were cultured in normal medium containing 10% newborn calf serum supplemented with (d) or without (a–c) 20 μM ethanolamine at 39.5°C for 3 d. B, Flow cytometric analyses of the DNA contents. CHO-K1 and mutant cells were cultured in ethanolamine-deficient medium supplemented with (R-41+Etn) or without (CHO-K1, R-41) 20 μM ethanolamine at 39.5°C for 4 d. CHO-K1: G₁, 41.6%; S, 49.1%; G₂/M, 9.3%. R-41: G₁, 38.9%; S, 16.3%; G₂/M, 44.8%. R-41+Etn: G₁, 38.2%; S, 50.5%; G₂/M, 11.3%. Bars, 30 μm.