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. 2000 Jun 12;149(6):1249–1262. doi: 10.1083/jcb.149.6.1249

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9907095.f4a.jpg — PYK-2 and the MTOC are translocated to the area of cell–cell contact of NK cells with target cells during specific recognition and cytotoxicity. (a) Confocal sections showing PYK-2 localization on NK cell clones (E) which are inhibited (CD94/NKG2A), or activated (CD94/NKG2C) by HLA-E, and that were interacting with HLA-E⁻ (.221) or HLA-E⁺ (AEH) target cells (T). Cell–cell conjugates were stained for PYK-2 (green) and CD94 (red) and analyzed using confocal microscopy. Confocal sections under brightfield illumination are shown for each conjugate. Arrowheads show the localization of PYK-2 on effector cells. (b) MTOC localization on NK cell clones that are inhibited (CD94/NKG2A), or activated (CD94/NKG2C) by HLA-E, and that were interacting with HLA-E⁻ (.221) or HLA-E⁺ (AEH) target cells. Cell conjugates stained for γ-tubulin were photographed under epifluorescence (red) and brightfield conditions using a Nomarski 60× objective. T and E indicate target and effector cells, respectively, while arrowheads point to the MTOC on effector cells.

graphic file with name JCB9907095.f4b.jpg — PYK-2 and the MTOC are translocated to the area of cell–cell contact of NK cells with target cells during specific recognition and cytotoxicity. (a) Confocal sections showing PYK-2 localization on NK cell clones (E) which are inhibited (CD94/NKG2A), or activated (CD94/NKG2C) by HLA-E, and that were interacting with HLA-E⁻ (.221) or HLA-E⁺ (AEH) target cells (T). Cell–cell conjugates were stained for PYK-2 (green) and CD94 (red) and analyzed using confocal microscopy. Confocal sections under brightfield illumination are shown for each conjugate. Arrowheads show the localization of PYK-2 on effector cells. (b) MTOC localization on NK cell clones that are inhibited (CD94/NKG2A), or activated (CD94/NKG2C) by HLA-E, and that were interacting with HLA-E⁻ (.221) or HLA-E⁺ (AEH) target cells. Cell conjugates stained for γ-tubulin were photographed under epifluorescence (red) and brightfield conditions using a Nomarski 60× objective. T and E indicate target and effector cells, respectively, while arrowheads point to the MTOC on effector cells.