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. 2000 Jun 26;149(7):1433–1442. doi: 10.1083/jcb.149.7.1433

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Endogenous dishevelled protein is present in multiple subcellular locations in embryonic kidney mesenchymal and epithelial cells. (A–C) Endogenous dishevelled and E-cadherin staining in E12 mouse kidneys cultured ex vivo for 3 d and fixed in 2% paraformaldehyde. Diffuse dvl is present throughout the cytoplasm. Dvl concentrates at lateral membranes (large arrowheads) and apical cell surfaces (arrows). Punctate dvl staining is present apically (arrows) and basally (small arrowheads). (D–F) Dvl staining partially overlaps with the rhodamine-phalloidin signal associated with filamentous actin along the basal (small arrowheads), lateral (large arrowheads), and apical (arrow) surfaces.