Figure 3.

Patch clamp recordings of Na⁺ inward currents recorded from neurons in N/A cocultures 4 d (A₁ and A₂) and 10 d (B₁ and B₂) after neuronal plating. A₁ and B₁, Superimposed traces of currents in response to depolarizing pulses of +70, +80, +90, and +100 mV stepped from a holding potential of –100 mV. A₂–B₂, Occurrence of spontaneous synaptic inward currents illustrated by three consecutive current traces recorded at a holding potential of –60 mV. Current traces shown in A₁ and A₂ and B₁ and B₂ were from the same cells and were obtained with pipettes filled with a CsCl solution.