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. 2000 Apr 17;149(2):447–456. doi: 10.1083/jcb.149.2.447

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© 2000 The Rockefeller University Press

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graphic file with name JCB9906130.f6a.jpg — Bcl-X_L is not downregulated in suspension in the IEC-18 variant AR 1.10 that spontaneously acquired resistance to anoikis. (A) Analysis of apoptosis by cell death ELISA in IEC-18 and AR 1.10 cells that were cultured in suspension. Results represent the average of two independent experiments plus the SD. (B) Western blot analysis of Bcl-X_L in AR 1.10 cells that were cultured in monolayer (mon) or in suspension (susp) for the indicated time periods. The membrane was reprobed with an anti-CDK4 antibody as a loading control.

graphic file with name JCB9906130.f6b.jpg — Bcl-X_L is not downregulated in suspension in the IEC-18 variant AR 1.10 that spontaneously acquired resistance to anoikis. (A) Analysis of apoptosis by cell death ELISA in IEC-18 and AR 1.10 cells that were cultured in suspension. Results represent the average of two independent experiments plus the SD. (B) Western blot analysis of Bcl-X_L in AR 1.10 cells that were cultured in monolayer (mon) or in suspension (susp) for the indicated time periods. The membrane was reprobed with an anti-CDK4 antibody as a loading control.