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. 2000 Apr 17;149(2):447–456. doi: 10.1083/jcb.149.2.447

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© 2000 The Rockefeller University Press

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graphic file with name JCB9906130.f7a.jpg — Enforced downregulation of Bcl-X_L in the ras-transformed cells with the antisense oligodeoxyribonucleotide results in the inhibition of ras-induced resistance to anoikis. (A) Western blot analysis of Bcl-X_L in mock-transfected IEC-ras-3 cells, IEC-ras-3 cells transfected with a control, or antisense Bcl-X_L oligonucleotides. The membrane was reprobed with anti-CDK 4 antibody as a loading control. (B) Analysis of apoptosis by cell death ELISA in mock-transfected IEC-ras-3 cells, IEC-ras-3 cells transfected with a control, or antisense Bcl-X_L oligonucleotides that were cultured in monolayer or suspension overnight. Results represent the average of duplicates plus the SD. Induction of anoikis was calculated as the ratio of the apoptotic signal observed in suspension versus the monolayer. This experiment was performed three times with similar results.

graphic file with name JCB9906130.f7b.jpg — Enforced downregulation of Bcl-X_L in the ras-transformed cells with the antisense oligodeoxyribonucleotide results in the inhibition of ras-induced resistance to anoikis. (A) Western blot analysis of Bcl-X_L in mock-transfected IEC-ras-3 cells, IEC-ras-3 cells transfected with a control, or antisense Bcl-X_L oligonucleotides. The membrane was reprobed with anti-CDK 4 antibody as a loading control. (B) Analysis of apoptosis by cell death ELISA in mock-transfected IEC-ras-3 cells, IEC-ras-3 cells transfected with a control, or antisense Bcl-X_L oligonucleotides that were cultured in monolayer or suspension overnight. Results represent the average of duplicates plus the SD. Induction of anoikis was calculated as the ratio of the apoptotic signal observed in suspension versus the monolayer. This experiment was performed three times with similar results.