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. 2000 Apr 17;149(2):307–316. doi: 10.1083/jcb.149.2.307

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9908117.f3a.jpg — OSM induces hnRNP A1 phosphorylation. Metabolically ³²P-labeled NIH-3T3 cells were either left untreated or were exposed to OSM (DME + 400 mM sorbitol (A) or DME + 600 mM sorbitol (B)) for the indicated times. Total protein was isolated, endogenous hnRNP A1 was immunoprecipitated with the monoclonal antibody 4B10, and its phosphorylation level was determined by autoradiography following SDS-PAGE. Western blotting analysis using the 4B10 antibody showed comparable amounts of hnRNP A1 protein present in all lanes (lower panel in A and B). Exposure of NIH-3T3 cells to 600 mM sorbitol resulted in cytoplasmic accumulation of hnRNP A1 in ∼100% of the cells after 2 h, and hnRNP A1 cytoplasmic localization was not reversible after 5 h.

graphic file with name JCB9908117.f3b.jpg — OSM induces hnRNP A1 phosphorylation. Metabolically ³²P-labeled NIH-3T3 cells were either left untreated or were exposed to OSM (DME + 400 mM sorbitol (A) or DME + 600 mM sorbitol (B)) for the indicated times. Total protein was isolated, endogenous hnRNP A1 was immunoprecipitated with the monoclonal antibody 4B10, and its phosphorylation level was determined by autoradiography following SDS-PAGE. Western blotting analysis using the 4B10 antibody showed comparable amounts of hnRNP A1 protein present in all lanes (lower panel in A and B). Exposure of NIH-3T3 cells to 600 mM sorbitol resulted in cytoplasmic accumulation of hnRNP A1 in ∼100% of the cells after 2 h, and hnRNP A1 cytoplasmic localization was not reversible after 5 h.