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. 2000 Apr 17;149(2):307–316. doi: 10.1083/jcb.149.2.307

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9908117.f6a.jpg — Activation of the MKK_3/6-p38 pathway is sufficient to promote cytoplasmic accumulation of hnRNP A1. (a) COS cells cultured on glass coverslips were transiently transfected with 5 μg of expression plasmids encoding myc-tagged versions of the MKK_3/6 kinase (data not shown) or its permanently active mutant (MKK_3/6 ^DD), in conjunction with 5 μg of expression plasmids encoding HA-tagged versions of wild-type p38 kinase or its dominant-negative mutant (p38^DN). The cells were then fixed and stained with the mouse monoclonal antibody 4B10 and FITC-conjugated anti–mouse IgG (green) to detect endogenous hnRNP A1 protein, and with the rabbit polyclonal anti-myc antibody and rhodamine-conjugated anti–rabbit IgG (red) to detect the transiently expressed MKK_3/6 ^DD _. (b) Histogram showing the percentage of cells displaying cytoplasmic hnRNP A1 accumulation after transfection as described in a.

graphic file with name JCB9908117.f6b.jpg — Activation of the MKK_3/6-p38 pathway is sufficient to promote cytoplasmic accumulation of hnRNP A1. (a) COS cells cultured on glass coverslips were transiently transfected with 5 μg of expression plasmids encoding myc-tagged versions of the MKK_3/6 kinase (data not shown) or its permanently active mutant (MKK_3/6 ^DD), in conjunction with 5 μg of expression plasmids encoding HA-tagged versions of wild-type p38 kinase or its dominant-negative mutant (p38^DN). The cells were then fixed and stained with the mouse monoclonal antibody 4B10 and FITC-conjugated anti–mouse IgG (green) to detect endogenous hnRNP A1 protein, and with the rabbit polyclonal anti-myc antibody and rhodamine-conjugated anti–rabbit IgG (red) to detect the transiently expressed MKK_3/6 ^DD _. (b) Histogram showing the percentage of cells displaying cytoplasmic hnRNP A1 accumulation after transfection as described in a.