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. 2000 Apr 17;149(2):307–316. doi: 10.1083/jcb.149.2.307

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9908117.f7a.jpg — Activation of the MKK_3/6-p38 pathway and the concomitant reduction of the level of nuclear hnRNP A1 correlates with changes in alternative splicing. COS cells were transiently transfected with 6 μg of plasmid encoding the E1A splicing reporter minigene alone or together with 7 μg of expression plasmids encoding myc-tagged versions of the MKK_3/6 kinase, or its permanently active mutant (MKK_3/6 ^DD), in conjunction with 7 μg of expression plasmids encoding HA-tagged versions of wild-type p38 kinase or its dominant-negative mutant (p38^DN). At 24 h after transfection cells were either left untreated or exposed to 600 mM sorbitol for 4 h. Total RNA was then isolated and the alternative splicing pattern of the E1A transcripts was determined by RT-PCR. The relative levels of 13S, 12S, and 9S mRNAs were quantitated as described (Cáceres et al. 1994; Screaton et al. 1995). The E1A transcript isoforms are shown schematically. Essentially identical results were obtained in three independent experiments.

graphic file with name JCB9908117.f7b.jpg — Activation of the MKK_3/6-p38 pathway and the concomitant reduction of the level of nuclear hnRNP A1 correlates with changes in alternative splicing. COS cells were transiently transfected with 6 μg of plasmid encoding the E1A splicing reporter minigene alone or together with 7 μg of expression plasmids encoding myc-tagged versions of the MKK_3/6 kinase, or its permanently active mutant (MKK_3/6 ^DD), in conjunction with 7 μg of expression plasmids encoding HA-tagged versions of wild-type p38 kinase or its dominant-negative mutant (p38^DN). At 24 h after transfection cells were either left untreated or exposed to 600 mM sorbitol for 4 h. Total RNA was then isolated and the alternative splicing pattern of the E1A transcripts was determined by RT-PCR. The relative levels of 13S, 12S, and 9S mRNAs were quantitated as described (Cáceres et al. 1994; Screaton et al. 1995). The E1A transcript isoforms are shown schematically. Essentially identical results were obtained in three independent experiments.