Figure 3.

Cell adhesion defects of T1385A and N1386A mutants. We tested cell adhesion to plates coated with two concentrations of wild-type and mutant FN7–10: 20 μg/ml (high FN) or 0.8 μg/ml (low FN). Adhesion to each mutant protein was normalized to the wild type at each coating concentration; adhesion to 0.8 μg/ml wild-type FN7–10 was ∼70% of adhesion to 20 μg/ml. The low concentration of FN gave a more sensitive indication of adhesion defects.