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. 2000 Apr 17;149(2):521–527. doi: 10.1083/jcb.149.2.521

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Gradient sedimentation analysis of FN7–10 mutant proteins. The indicated proteins were sedimented through 15–40% glycerol gradients, and the fractions were analyzed by SDS-PAGE. Ovalbumin (OVA, 3.5 S) and bovine serum albumin (BSA, 4.6 S) were used as standards. Lanes are labeled as follows: ST, starting material; 5–10, fractions 5–10; and M, markers (with size indicated on the lower left gel). The 30–40% recovery of protein is typical for this type of gradient because of the loss on the walls of the tubes.

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