Figure 7.

Analysis of the amounts of CP26 in thylakoids isolated from leaves at different stages. UY, Upper leaves (harvested from the top of young plants of ∼30-cm height); LY, lower leaves from the same plants; MM, fully expanded leaves (harvested from the middle parts of mature plants at the time of flowering); LM, older leaves (harvested from the lower parts of the same plants). A, Coomassie staining of isolated thylakoid proteins separated in a 10% PAA gel. Whereas CP26 is clearly detectable in very young leaf material of the ycf9 knockout plant (9-1), it is practically undetectable in older leaves. WT, Wild-type; M, molecular weight marker. B, Western blot analysis using an anti-CP26 antibody. The analysis confirms virtually complete lack of CP26 in mature leaf tissue of ycf9 knockout plants. The CP29 protein cross-reacting with this antibody (Di Paolo et al. 1990; Fig. 6 B) is also indicated.