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. 2000 Apr 17;149(2):255–262. doi: 10.1083/jcb.149.2.255

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Detection of cytoplasmic inclusions formed by ARdNLSQ1, ARdNLSQ22, and ARdNLSQ77 in COS-7 cells. COS-7 cells were transfected with the indicated receptor constructs lacking the NLS, treated with (D–O) or without (A–C) DHT, and the cellular localization of the receptors assessed by fluorescence microscopy. Shown are the different types of aggregates formed as detected by immunofluorescence microscopy, DIC images, and an overlay of the two pictures. Bars: 15 μm (A–C); 30 μm (D–L); and 15 μm (M–O).