Table I.
Number of Clonotypes Represented in TIL at:
|
||||||
---|---|---|---|---|---|---|
Total Number of TRBV Sequences Analyzed | Number of Distinct Clonal Sequencesa | <5% | 5–9% | 10–20% | >20% | |
Responder | ||||||
6 | 118 | 41 | 37 | 1 | 3 | 0 |
9 | 128 | 10 | 4 | 2 | 2 | 2 |
10 | 92 | 20 | 17 | 0 | 0 | 3 |
16 | 64 | 20 | 16 | 2 | 0 | 2 |
17 | 81 | 24 | 21 | 0 | 2 | 1 |
19 | 78 | 38 | 32 | 4 | 2 | 0 |
21 | 91 | 37 | 32 | 2 | 3 | 0 |
25 | 81 | 39 | 35 | 3 | 1 | 0 |
26 | 84 | 37 | 32 | 3 | 1 | 1 |
28 | 78 | 15 | 12 | 1 | 0 | 2 |
30 | 81 | 37 | 33 | 0 | 3 | 1 |
31 | 75 | 18 | 13 | 4 | 0 | 1 |
34 | 80 | 21 | 19 | 1 | 0 | 1 |
Nonresponder | ||||||
7 | 80 | 34 | 31 | 2 | 0 | 1 |
11 | 82 | 4 | 3 | 0 | 0 | 1 |
12 | 89 | 78 | 78 | 0 | 0 | 0 |
13 | 70 | 22 | 18 | 1 | 2 | 1 |
14 | 85 | 3 | 1 | 0 | 1 | 1 |
15 | 67 | 14 | 10 | 2 | 1 | 1 |
18 | 61 | 22 | 17 | 3 | 1 | 1 |
20 | 86 | 34 | 32 | 0 | 1 | 1 |
22 | 81 | 15 | 10 | 1 | 2 | 2 |
24 | 85 | 23 | 19 | 1 | 1 | 2 |
27 | 73 | 26 | 23 | 1 | 1 | 1 |
29 | 78 | 21 | 17 | 1 | 1 | 2 |
Clonotypes present within TIL samples were identified by comparison of the TRBV, D, and J region sequences of cDNA clones that were derived from individual TRBV genes.