Table III.
Ag reactivity of TIL and persistent T
| Patient No. | Ag Reactivity of TILa | Reactivity of Most Prevalent Persistent Clonotypeb | Most Prevalent Persistent Clonotype (%) |
|---|---|---|---|
| Responder | |||
| 6 | Autol. | Autol. | 16 |
| 9 | MART-1 | MART-1 | 83 |
| 10 | MART-1 | MART-1 | 77 |
| 16 | Autol. | Autol. | 12 |
| 17 | Autol. | Autol. | 8 |
| 19 | Autol. | N/Tc | 5 |
| 21 | Autol. | Autol. | 8 |
| 25 | Autol. | N/T | 7 |
| 26 | MART-1 | - | 1 |
| 28 | MART-1 | MART-1 | 7 |
| 30 | gp100:209–217 | Autol. | 7 |
| 31 | MART-1 | - | 2 |
| 34 | Autol. | N/T | 43 |
| Nonresponder | |||
| 7 | MART-1 | - | <1 |
| 11 | MART-1 | - | <1 |
| 12 | Autol. | - | 4 |
| 13 | MART-1 | - | 1 |
| 14 | Autol. | - | <1 |
| 15 | MART-1 | - | 4 |
| 18 | gp100:209–217 | - | <1 |
| 20 | MART-1 | - | 2 |
| 22 | Autol. | - | 3 |
| 24 | Autol. | N/T | 17 |
| 27 | MART-1 | - | 1 |
| 29 | MART-1 | - | <1 |
Ag reactivity was evaluated by determining the ability of TIL to release IFN-γ in response to autologous (Autol.) or HLA-matched allogeneic tumors, or to T2 cells that were pulsed with either the MART-1:26–35 or gp100:209–217 HLA-A2-restricted peptides.
The Ag reactivity of the most persistent clonotype detected in PBMC 23–63 days after transfer is indicated. Isolated T cell clones corresponding to the dominant persistent clonotypes from TIL from patients 9 and 10 released IFN-γ in response to T2 cells that were pulsed with the MART-1:27–35 peptide, and clones corresponding to the dominant persistent clonotypes within TIL from patients 6, 16, and 22, released IFN-γ in response to autologous tumor cells. The dominant persistent clonotype within the TIL from patient 28 was identified by staining with anti-TRBV Abs and a MART-1:26–35(2L) tetramer. Tumor reactivity of the dominant persistent clonotypes from patients 17 and 30 was determined by measuring the ability of these T cells, which were identified within the TIL using specific TRBV Abs, to specifically up-regulate expression of cell surface CD107a expression following stimulation with autologous tumor cells. A dash indicates either that no persistent clonotype was detected or that the most persistent clonotype was present at a frequency of between 1 and 5% and was not evaluated due to the limited number of cells available for analysis.
For samples designated N/T (not tested), the ability of the persistent clonotype to recognize autologous tumor has not been verified.