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. Author manuscript; available in PMC: 2008 Jan 7.
Published in final edited form as: J Immunother. 2005;28(1):53–62. doi: 10.1097/00002371-200501000-00007

FIGURE 7.

FIGURE 7

Determination of tumor antigen reactive T-cell clones and persistence of 2098BV12-4a T-cell clone in melanoma patient 2098. A, 1 × 105 cloned 2098BV2a, 2098BV7-9a, 2098BV12-4a, or 2098BV27 T cells were co-cultured for 16 hours with 1 × 105 2098mel tumor cells or 1978EBV-B cells pulsed with no peptide, 1 μM GAS7-10merMut, or 1 μM GAPDH-10merMut for 2 hours. The concentration of IFN-γ in the culture medium released by T cells was measured by ELISA assay. B, TRBV12-4 sequences were amplified from TIL 2098 as well as PBMC1w, PBMC8w, and PBMC32w samples obtained at 1, 8, and 32 weeks respectively following adoptive TIL 2098 transfer by RT-PCR using specific TRBV12-4 gene family primers. The percentage of TRBV12-4a sequences was normalized by FACS analysis in the total populations.