Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Aug 7;150(3):527–538. doi: 10.1083/jcb.150.3.527

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

COOH-terminal ActA truncations. (a) Schematic diagram of secreted 6xHis-tagged ActA and sequence alignments with WASP family proteins and cofilin. The signal sequence is labeled SS. Regions within the mature NH₂ terminus are labeled as follows: acidic (A), actin binding (AB), and cofilin homology (C). The proline-rich repeats are shaded black, and the 6xHis tag is shaded in gray. (b) Diagram of derivatives of ActA that were truncated at amino acids 263 (A263), 201 (A201), 165 (A165), 135 (A135), and 101 (A101). (c) Purified-truncated derivatives of ActA visualized on a 15% polyacrylamide gel stained with Coomassie blue. The leftmost lane contains molecular weight markers (MWM).