Table 1.
Primers and Strains Used in this Study
| ActAconstruct | Primer | 6xHis-taggedsecreting strain | 10403S isogenic mutant strain |
|---|---|---|---|
| ActA | (Welch et al. 1998) | DP-L2723 | – |
| A263 | (Welch et al. 1998) | DP-L4007 | – |
| A201 | 5′-ATGCCCGGGTGTAGACTCGTCTGCTGA-3′ | DP-L3939 | – |
| A165 | 5′-ATGCCCGGGATAAGTAAGGCTTTCAAG-3′ | DP-L3942 | – |
| A135 | 5′-ATGCCCGGGATGACGACGCTCCACTTG-3′ | DP-L3944 | – |
| A101 | 5′-ATGCCCGGGACCTTTCTCTGCTTTTGC-3' | DP-L3946 | – |
| Δ31-262 | 5′-CCCGACATAATATTTGCAGCTAGCGACTTCCCGCCACCACCTACGGAT-3′ | DP-L3966 | – |
| Δ31-165 | 5′-CCCGACATAATATTTGCAGCGGATAAACCAACAAAAGCAAAT-3′ | DP-L3968 | DP-L3984 |
| Δ202-263 | 5′-TCAGCAGACGAGTCTACACCAGACTTCCCGCCACCACCTACG-3′ | DP-L3964 | DP-L3982 |
| Δ136-200 | 5′-CAAGTGGAGCGTCGTCATCCACCACAACCTTTAAAAGCAAATC-3′ | DP-L3972 | DP-L3988 |
| Δ60-165 | 5′-AGCGAGGTAAATACGGGACCAGATAAACCAACAAAAGCAAATAAG-3′ | DP-L3970 | DP-L3986 |
| Δ136-165 | 5′-CAAGTGGAGCGTCGTCATCCAGATAAACCAACAAAAGCAAAT-3′ | DP-L3974 | DP-L3990 |
| Δ146-150 | 5′-TCGGATAGCGCAGCGGAAATTGCCATAGCGTCGTCGGATAGT-3′ | DP-L3976 | DP-L3992 |
| Δ60-101 | 5′-AGCGAGGTAAATACGGGACCAAATAACAATAATAACAACGGTGAG-3′ | DP-L3978 | DP-L3994 |
| Δ31-58 | 5′-CCCGACATAATATTTGCAGCGCCAAGATACGAAACTGCACGT-3′ | DP-L3980 | DP-L3996 |
| Δ7-633 | 5′-ATTTTTTCTTAATTGAAATCTATTTAATCC-3′ | DP-L3078 |