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Expressed nebulin SH3 domain does not cosediment in the presence of F-actin and α-actinin. (A) F-α-actin, (B) nebulin SH3, (C) F-α-actin + nebulin SH3, (D) α-actinin, (E) F-α-actin + α-actinin, and (F) F-α-actin + α-actinin + nebulin SH3. P, pellet; S, supernatant; M, molecular marker.
