Table 1.
Variable Composition of Double-stained MYC/nebulin Ectopic Fibrils/Patches
MYC-positive | Rho-phalloidin–positive | c-α-actin–positive | Nebulin (NB2)-positive | Tropomyosin-positive | S-α-actinin–positive | Titin (Z1Z2)-positive | MHC-positive |
---|---|---|---|---|---|---|---|
100% | 93% | 67% | 60% | 37% | 3% | 0% | 0% |
(n = 210) | (n = 30) | (n = 30) | (n = 30) | (n = 30) | (n = 30) | (n = 30) | (n = 30) |
Day 10 cultures expressing MYC/M171-M183 were double stained with anti-MYC and Rho-phalloidin, or with antibodies to other myofibrillar proteins. A total of 210 randomly selected MYC-positive fibrils/patches were examined, and the percentage that costained for the other myofibrillar proteins determined.