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. 2000 Aug 7;150(3):433–446. doi: 10.1083/jcb.150.3.433

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Behavior of NDF during telophase in living cells. The CMT3 cells were transiently transfected with the protein B23-GFP vector. After 24 h, cells in telophase were selected for observation by time-lapse confocal fluorescence microscopy. The images were collected every 18 s over a 90-min period. See also video 1 available at http://www.jcb.org/cgi/content/full/150/3/433/DC1. Protein B23-GFP was present in several NDF in the cytoplasm and distributed throughout the nucleoplasm and in newly forming nucleoli. The disappearance of the NDF in the proximity of the nuclear envelope (arrows) was accompanied by a concomitant increase in the fluorescence of the adjacent nuclear interior (arrowheads). Bar, 2 μm.