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. 2000 Aug 7;150(3):499–512. doi: 10.1083/jcb.150.3.499

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Anti-KRP₁₈₀ antibodies specifically recognize this motor in sea urchin egg extracts. A recombinant GST-KRP₁₈₀ COOH-terminal coiled-coil fusion protein was purified on a glutathione–Sepharose column (lane GST-Coil 2) and used to raise mouse polyclonal antisera. The fusion protein runs at 60 kD. Affinity-purified anti-KRP₁₈₀ antibodies from four different mice all specifically recognize a polypeptide ∼180 kD in mass (lanes 180.1, 180.2, 180.3, and 180.4) found in sea urchin high speed supernatant (HSS) unfertilized egg extract (lane Egg HSS; Coomassie-stained SDS-7.5% PAGE).