Table 2.
Spindle Dynamics in Antibody and Recombinant Protein-injected Embryos
| Reagent | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mouse IgG | n | Anti-KRP180 | n | GST | n | GST-Coil 1 | n | GST-Coil 2 | n | |
| Average velocity | μm/min | |||||||||
| Rate of slow phase | 1.08 ± 0.45 | 5 | 1.06 ± 0.06 | 3 | 1.41 ± 0.32 | 4 | 1.30 ± 0.1 | 5 | 1.04 ± 0.38 | 9 |
| Rate of fast phase | 3.55 ± 0.38 | 5 | 3.22 ± 0.60 | 7 | 3.80 ± 0.75 | 4 | 3.90 ± 0.97 | 5 | 3.37 ± 0.53 | 10 |
| Rate of spindle collapse | NA | 2.00 ± 0.41 | 4 | NA | NA | 0.87 ± 0.2 | 7 | |||
| Average time | min | |||||||||
| Initiation of anaphase | 14.6 ± 4.3 | 6 | 16.0 ± 3.2 | 6 | 12.8 ± 4.3 | 4 | 13.0 ± 3.5 | 5 | 15.2 ± 3.5 | 8 |
| Duration of slow phase | 12.2 ± 1.9 | 6 | 10.5 ± 1.3 | 3 | 11.4 ± 2.9 | 4 | 13.3 ± 3.3 | 5 | 12.5 ± 1.9 | 9 |
| Duration of fast phase | 8.8 ± 1.7 | 6 | 11.3 ± 4.8 | 7 | 7.3 ± 2.5 | 3 | 8.3 ± 2.5 | 4 | 7.8 ± 1.8 | 9 |
NA, Not applicable because this event did not occur.